This course provides students with a sound practical and theoretical knowledge of key techniques perform cell culture. Topics include growth environment, routine maintenance of cell cultures, cryopreservation, transfection, and cell counting. Other topics include cloning techniques, suspension culture, serum-free culture, evaluation of cryopreserved cells, extraction of DNA from mammalian cells, and use of multi-well plates in cell culture. Upon completion, students should be able to demonstrate the knowledge and skills required to initiate, maintain, and manipulate cells in culture. Internship opportunity available.
This course is designed for Lab Technicians, Associate Scientists, Research and Development Personnel, as well as professional in the biotechnology and life science seeking training and hands-on experience in cell and tissue culture.