Capillary Electrophoresis as a Complement to Mass Spec for Protein Characterization

Description

Summary: Characterization of therapeutic proteins relies increasingly on the use of mass spectrometry.  But the complex nature of proteins sometimes warrants alternative approaches to provide an orthogonal perspective.  One tool that can complement mass spectrometry is capillary electrophoresis (CE).  The method springs from gel electrophoresis, one of the earliest protein analytical techniques and one still used today, but the introduction of CE allows for smaller sample amounts, reduced prep time, speedier analysis,  and better control.  Modern CE can be used as an alternative to mass spectrometry and can also be used in conjunction with mass spectrometry.  We'll look at some of the key uses of CE in protein analysis.
 
Presenter: Mike Molloy, Technical director of the Biologics Laboratory & Karina Allen-Ludwig, Senior Scientist in the Biologics Laboratory 
 
Mike's Bio: Mike Molloy has been with Boston Analytical since 2012, serving first as the Director of the QC Chemistry laboratory, then heading Analytical Development in Chemistry and most recently serving as the Technical director overseeing the Biologics Laboratory. Prior to joining Boston Analytical, he spent twenty years in pharmaceutical development at Biogen, developing bioassays for product characterization and methods for bioanalysis of clinical trial  pharmacokinetics samples. Molloy was head of laboratory operations in the Preclinical and Clinical Developments Sciences group at Biogen. Mike holds a master’s degree in biology from Umass Lowell and a bachelor’s in biology from UNH.
 
Karina's Bio:  Dr. Karina Allen-Ludwig, is the Senior Scientist in the Biologics Laboratory at Boston Analytical since 2017 and manages Method development for quantitative and qualitative analysis of pharmaceutical products according to FDA and cGMP guidelines. Karina has extensive experience in the analytics of biological macromolecules from small peptides to complex proteins. Prior to Boston Analytical, Karina was the lead chemist/scientist for a small start-up company that performed research work for NIDA and the DEA on the isolation, analysis and purification of cannabinoids from cannabis sativa biomass. Karina holds a Ph.D from the University of Massachusetts Lowell- Kennedy College of Sciences in Chemistry- with a focus on Biochemistry.